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2 edition of Genetic and molecular analysis of the gai, ga4 and fhy2 mutations of arabidopsis. found in the catalog.

Genetic and molecular analysis of the gai, ga4 and fhy2 mutations of arabidopsis.

Jinrong Peng

Genetic and molecular analysis of the gai, ga4 and fhy2 mutations of arabidopsis.

by Jinrong Peng

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Published by University ofEast Anglia in Norwich .
Written in English


Edition Notes

Thesis (Ph.D.), University of East Anglia, School of Biological Sciences, 1993.

ID Numbers
Open LibraryOL21847154M

The physiological and molecular genetic studies as well as microarray and whole transcriptome studies have revealed a wide range of interactions between ethylene and auxin, cytokinins Background and Aims The occurrence of Arabidopsis thaliana semi-dwarf accessions carrying inactive alleles at the gibberellin (GA) biosynthesis GA5 locus has raised the question whether there are pleiotropic effects on other traits at the root level, such as rooting depth. In addition, it is unknown whether semi-dwarfism in arabidopsis confers a growth advantage under water-limiting conditions

A forward genetic screen identified RNA polymerase II‐associated factor 1 (Paf1/VIP3) that was required for some aspects of thigmomorphogenesis and also was defective in the rapid induction of TOUCH3 (TCH3) and TOUCH4 (TCH4), providing a link between rapid molecular responses to a single touch stimulus and growth alteration to repeated Analysis of an activation‐tagged GA‐deficient mutant, ddf1 (dwarf and delayed‐flowering 1), revealed that overexpression of DDF1, which encodes an AP2 transcriptional factor of the dehydration‐responsive element binding protein (DREB1)/C‐repeat binding factor (CBF) subfamily, reduces bioactive GAs in Arabidopsis (Magome et al., ).

Plants with shorter internodes are suitable for high-density planting, lodging resistance and the preservation of land resources by improving yield per unit area. In this study, we identified a locus controlling the short internode trait in watermelon using Zhengzhouzigua (long internode) and Duan (short internode) as mapping parents. Genetic analysis indicated that F1 plants were Ishfaq Majid, Nazia Abbas, in Senescence Signalling and Control in Plants, Gibberellic Acid. Gibberellic acid (GA) is an important senescence-impeding hormone whose active form declines in leaves as a plant ages (Schippers et al., ).The prominent role of GA in leaf senescence is largely unexplored, but limited studies regarding the negative regulators of GA-signaling pathways (i


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Genetic and molecular analysis of the gai, ga4 and fhy2 mutations of arabidopsis by Jinrong Peng Download PDF EPUB FB2

Genetic and molecular analysis of the gai, ga4 and fhy2 mutations of Arabidopsis. (Thesis) Peng J. Publisher: University of East Anglia [] Metadata Source: The British Library Type: Thesis. Abstract. Highlight Terms No biological terms identified No abstract supplied. Genetic and molecular analysis of the gai, ga4 and fhy2 mutations of Arabidopsis.

Author: Peng, Jinrong. ISNI: Awarding Body: University of East Anglia Current Institution: University of East Anglia Date of Award: Availability of Full Text: ?uin= Identification of Insertion Mutations of the GID1a, GID1b, and GID1c Genes.

We investigated the physiological roles of the three Arabidopsis GID1 genes further by identifying and characterizing T-DNA and transposon insertion mutants for each gene (see Supplemental Figure 1A online).

We identified two independent insertion alleles of the GID1a gene, designated gid1a-1 and Analysis of the GA content of the ga4 and ga5mutants indicated that they were defective in GA 3β-hydroxylase and GA oxidase activity, respectively (Talon et al., ).

This was confirmed by the cloning of these loci. The GA4 locus was isolated after it was tagged by a chance T-DNA insertion (Chiang et al., ).

Ga4 and fhy2 mutations of arabidopsis. book gene contained an open   1. Introduction. Watermelon (Citrullus lanatus (Thumb) Matsum and Nakai) belongs to the genus Citrullus, is one of the most important horticultural crop in the world [1,2,3,4].The genus Citrullus includes about genera and species for diploid species (2n = 22), which are grown in Africa, Asia and in the Mediterranean region [4,5].Watermelon is an important member of Cucurbitaceae family In Arabidopsis, the DELLA subfamily of GRAS regulatory genes consists of GAI, RGA, RGA-LIKE1 (RGL1), RGL2, and RGL3.

GAI and RGA are known to be negative regulators of gibberellin (GA) responses. We found that RGL1 is a similar repressor of GA responses, as revealed by RGL1 gain-of-function and loss-of-function phenotypes. Repression of GA responses in Arabidopsis was conferred The gibberellins (GAs) are a complex family of diterpenoid compounds, some of which are potent endogenous regulators of plant growth.

As part of a feedback control of endogenous GA levels, active GAs negatively regulate the abundance of mRNA transcripts encoding GA biosynthesis enzymes. For example, Arabidopsis GA4 gene transcripts encode GA 3β-hydroxylase, an enzyme that catalyzes the Genetic and molecular studies have identified the GA receptors and several positive and negative components in the GA signaling cascade (Sun and Gubler, ; Hartweck and Olszewski, ).

Among them, three major players are the GA receptors, the DELLA repressor proteins, and the F-box proteins that control the stability of DELLA Bioactive gibberellins (GAs) are essential endogenous regulators of plant growth.

GA signaling is mediated via GAI, a nuclear member of the GRAS family of plant transcription factors. Previous experiments have suggested that GAI is a GA-derepressible repressor of plant growth.

Here we test this hypothesis by examining the effects of the expression of Arabidopsis GAI in transgenic Basmati A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the :// In Arabidopsis, seedless silique development or parthenocarpy can be induced by the application of various plant growth regulators (PGRs) to unfertilized pistils.

Ecotype-specific responses were observed in the Arabidopsis ecotypes Columbia and Landsberg relative to the type of PGR and level applied. The parthenocarpic response was greatest in ecotype Landsberg, and comparisons of fruit growth We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele (bri) of the brassinosteroid (BR) receptor BRI1.

The bri mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate the accumulation of mRNAs of the GA-responsive GASA1 gene, as well as the Recent molecular genetic and biochemical studies helped to identify GA biosynthesis and catabolism genes in Arabidopsis and other species.

A number of GA-deficient dwarf mutants in Arabidopsis were isolated by Maarten Koornneef and he named them ga1, ga2, ga3, ga4 Arabidopsis Delta-DELLA GAI (gai) was fused with a T7 epitope tag and expressed under the control of a companion cell-specific expression promoter, Commelina yellow mottle virus promoter (CoYMVp Erratum: Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis (Plant Cell () 18, ()) Article February with 23 Reads How we measure Li J, Wen J, Lease KA, Doke JT, Tax FE, Walker JC () BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling.

Cell – ; Liscum E, Briggs WR () Mutations in the NPH1 locus of Arabidopsis disrupt the perception of Arabidopsis mutants were employed to examine potential dependencies on gibberellin biosynthesis (ga, ga, and ga) and perception (spy-4 and gai) during parthenocarpic silique :// Bulley, S.

W., James, D. J., Phillips, A. and Hedden, P. Controlling stature in apple trees by genetic modification of gibberellin :// Genetic analysis of GA function has shown that in combination with testa mutants (such as tt4 and ttg1) the requirement of ga1 for GA is overcome (Debeaujon & Koornneef, ).

However, double‐mutant seeds still required light and chilling for radicle emergence, suggesting that GA is required to overcome the constraints of the testa, but also Rothamsted Repository. The gain‐of‐function gim2 mutant was insensitive to abscisic acid (ABA) in seed germination assays (A) Seeds of Col and gim2 were sown on germination medium (GM) containing 1% sucrose with (+E.) or without μM 17‐β‐estradiol and μM ABA.

The gim2 seed germination (determined by the radicle emergence) and post‐germination growth (determined by cotyledon greening) were Active gibberellins (GAs) are endogenous factors that regulate plant growth and development in a dose-dependent fashion.

Mutant plants that are GA deficient, or exhibit reduced GA responses, display a characteristic dwarf phenotype. Extragenic suppressor analysis has resulted in the isolation of Arabidopsis mutations, which partially suppress the dwarf phenotype conferred by GA deficiency and Rieu I, Eriksson S, Powers SJ, Gong F, Griffiths J, Woolley L et al (a) Genetic analysis reveals that CGA 2-oxidation is a major gibberellin inactivation pathway in Arabidopsis.

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